CCT4 and Zika virus infectious disease: To further investigate whether ZIKV infection alters bulk membrane protein synthesis, we pulse labelled mock-infected or ZIKV-infected wild-type and SRα/SRβ depleted cells with [35S]cysteine/methionine, then fractionated the lysates into cytosolic and membrane fractions and visualised by autoradiography (Fig 3C) or quantitated by scintillation counting (Fig 3D).