In conclusion, this study demonstrated that ZNF503-AS2 expression is elevated in glioma and has a good predictive ability for the prognosis of glioma patients, and the knockdown of ZNF503-AS2 can inhibit the proliferation, invasion, and migration of glioma cells, induce G2/M cell cycle arrest, and promote the apoptosis of glioma cells. Here, ZNF503 is linked to central nervous system cancer.