Moreover, IF analysis showed that PACAP38 treatment enhanced the localization of the SOX6 protein in nuclear, where serving as the site for transcription factors to regulate downstream gene expression (Figures 3D, E).Immunohistochemical analysis from the preceding animal experiments indicates that PACAP38 treatment enhances SOX6 expression in tumor tissues across different groups (Figure 3F). The gene discussed is ADCYAP1; the disease is neoplasm.