Aqueous ginseng extract has demonstrated an essential role in NK cell-mediated cytotoxicity through IFN-γ activation, while thymoquinone isolation from Nigella sativa increased NK cell cytotoxicity against MCF-7 breast cancer cells by expressing perforin, granzyme B, and IFN-α (Lucas et al., 2010). This evidence concerns the gene PRF1 and breast carcinoma.