It was found that the live birth rate was not significantly affected by the basic LH/FSH ratio in PCOS patients with a basal LH/FSH ratio ≥ 2 using different ovulation stimulation protocols by bicategorical logistic regression analysis after correcting for confounding factors, with an adjusted OR of 0.687, 95% CI [0.373–1.268] (Table S3, P = 0.230). The gene discussed is PLOD1; the disease is polycystic ovary syndrome.