To further explore the effect of Shikonin on necroptosis of glioma cells, we detected necroptosis-related proteins RIP1, RIP3, and MLKL expression levels of U251, U87, T98G cells treated with Shikonin (0, 4, 8, and 12 mM) for 24 hr. The gene discussed is RIPK1; the disease is glioma.