To further explore the effect of Shikonin on necroptosis of glioma cells, we detected necroptosis-related proteins RIP1, RIP3, and MLKL expression levels of U251, U87, T98G cells treated with Shikonin (0, 4, 8, and 12 mM) for 24 hr. Here, RIPK1 is linked to central nervous system cancer.