We have previously shown that CpG-Stat3 siRNA efficiently targeted A20 tumor cells to silence Stat3. 20,25 To evaluate gene silencing effect of CpG-Stat3 siRNA, we used quantitative real-time PCR (real-time qPCR) analysis of Stat3 mRNA of CpG-Stat3 siRNA-treated A20 tumor cells. This evidence concerns the gene STAT3 and neoplasm.