In a B16 melanoma model, blockade of VISTA altered the suppressive cellular profile of TME and reduced tumor-infiltrating MDSCs, while increasing the frequency of infiltrating effector T cells and enhancing the immunostimulatory phenotype of DCs with higher levels of expression of MHC II and CD80, and greater production of cytokines IL-12 and TNF-α [35]. The gene discussed is TNF; the disease is melanoma.