Of these, the combination with CA199 had significantly higher sensitivity (78%) and specificity (91%).29 Compared to ctDNA mutations, methylation of ctDNA showed a stronger potential in early diagnosis, and methylation of ADAMTS1 and BNC1 performed well in the early diagnosis of PDAC in terms of its sensitivity (97.4%), specificity (91.6%), and AUC (0.95).162 Although CA19-9 is a classical tumor marker, it lacks specificity in early diagnosis as CA19-9 lacks tumor specificity. This evidence concerns the gene BNC1 and neoplasm.