RA-FLSs were preincubated with the TLR2 antagonist TL2-C29, the TLR4 antagonist CLI-095 and the TLR9 antagonist ODN TTAGGG (ODN A151) for 1 h prior to the addition of spon-NETs and then further incubated for 24 h. Here, TLR2 is linked to rheumatoid arthritis.