CASP3 and neoplasm: Flow cytometry and IHC revealed a greater degree of tumor cell apoptosis in the S-HFD group than in the NFD group (Annexin V-positive rate: 26.6% vs. 30.27%, cleaved caspase-3-positive area: 0.56% vs. 1.0%; Fig. 2f, g; Supplementary Fig. 2f, g).