To examine the effects of EPHA3 mRNA regulation by VIM-AS1 on tumorigenesis and the tumor microenvironment in vivo, VIM-AS1-overexpressing and VIM-AS1- and EPHA3-overexpressing cell lines were subcutaneously injected into nude mice, and tumor growth curves and weights were monitored. The gene discussed is EPHA3; the disease is neoplasm.