Moreover, SIX1 (S225K) promoted liver cancer cell proliferation and invasion in vitro and growth and lung metastasis of liver tumor in vivo even more robustly than WT SIX1, while SIX1 (S225A) completely eliminated the capability of SIX1 to facilitate these effects (Fig. 5c–f, Supplementary Fig. S5c–f). The gene discussed is SIX1; the disease is liver cancer.