Multiple tools, including XCELL, TIMER, QUANTISEQ, MCPcounter, EPIC and CIBERSORT, were further utilized to evaluate the infiltration of these immunosuppressive cells in the HCC microenvironment, and the subsequential correlation analysis confirmed that some immunosuppressive subpopulations, such as M2-type TAMs and Th2 cells, were positively correlated with B4GALNT1 levels, whereas negative correlations were observed in CD8 + naive T cells that confer tumor-killing potentials (Xcell, R = −0.240, p < 0.001) (Fig. 5b). This evidence concerns the gene B4GALNT1 and hepatocellular carcinoma.