In this study, we found that the HuR protein expression was inducible in the mouse model of APAP‐induced liver injury and found that HuR could protect against APAP‐induced liver injury by promoting the expression of NRF2, cyclin A1, cyclin B1, cyclin D1, CDK2, ATG3, ATG5 and ATG7. This evidence concerns the gene ATG5 and injury.