PCSK1 and infection: To further clarify whether necroptosis impacts PPV infection, we pretreated the PTCs with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) for 4 h, respectively, and then infected the cells with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of the inhibitors.