Subsequently, we transfected porcine PTCs with equal amounts of PPV genome plasmids and initiation codon-mutated PPV genome plasmids, Western blot results demonstrated that the expression levels of ZBP1, p-RIPK3, and p-MLKL in the group transfected with the initiation codon-mutated PPV increased with prolonged infection time, exhibiting a trend consistent with the PPV-transfected group (Figure 6D). This evidence concerns the gene MLKL and infection.