The results showed that the levels of p-RIPK3 and p-MLKL were significantly increased in the ZBP1 overexpression PTCs comparing with the vector control transfected cells (Vec) during PPV infection, while the expression levels of RIPK3, MLKL, and Caspase-8 did not significantly change (Figure 2G). The gene discussed is ZBP1; the disease is infection.