ASXL1 and leukemia: No significant differences were identified between BOS and AML-ASXL1 variant allele frequency (VAF) for the pathogenic variant/s in ASXL1. AML-ASXL1 variants had a slightly lower average VAF compared with BOS due to the fact that AML samples are heterogeneous, harboring both leukemia cells with ASXL1 variants and non-leukemia cells which decreases the proportion of reads mapping to the ASXL1 variant.