Immunostaining of tumor tissues harvested from virgin KANK1-WTPyMT mice at different tumor stages revealed that non-transformed LECs lacked KANK1 expression (Supplementary Fig. 4e), whereas transformed, single-layered hyperplastic LECs exhibited KANK1 expression at their basal side and weak KANK1 signals at cell-cell junctions (arrowheads in Supplementary Fig. 4e), and LECs of multilayered carcinomas showed strong KANK1 signals at cell-cell junctions (Fig. 2e, asterisks in Fig. 2d and Supplementary Fig. 4e). This evidence concerns the gene KANK1 and neoplasm.