In line with the accelerated tumor growth, hematoxylin/eosin (H/E) sections of KANK1-WTPyMT tumors showed numerous small foci of carcinoma in situ at initial palpation, which increased in number and size at 16 weeks, and additionally, began to breach the basement membrane (BM) and invade the surrounding tissue (Supplementary Fig. 4c, d). This evidence concerns the gene KANK1 and in situ carcinoma.