DBP and infection: The time points post-infection selected represent an early time post-infection (16 hpi), when E2-DBP early RCs are readily detected as small, spherical, nuclear foci; an intermediate time point (24 hpi), when viral DNA replication reaches the maximum rate of synthesis and RCs display a combination of spherical and ring-like morphologies; and a late time point (36 hpi), when RCs coalesce and occupy a large proportion of the cell nucleus (22, 24, 36, 43).