CD4 and infection: Although healthy adults only had a difference in 1 cluster based on subsequent infection (Figure 5D), hierarchical gating revealed a trend towards higher levels of circulating plasmablasts and non-classical monocytes (Supplementary Figure S5C), with a deficit of subset 1 of CD4–CD8– T-cells (cluster 21 in Table 1) and a trend towards lower levels of subset 4 of TEMRA Tγδ cells (cluster 26 in Table 1) in subsequently infected individuals (Supplementary Figure S5D).