In particular, CD19-B220+CD5+CD1d- Bregs (with the highest expression of immunomodulatory molecules), CD19+B220+CD5+CD1d+ B10 Bregs, and CD19+B220+CD27+IgD+ NSw MBCs exhibited the most immunosuppressive phenotype in vivo based on their PD-L1 and IL-10 expression in murine HCC/CaMIN tissues. This evidence concerns the gene CD27 and hepatocellular carcinoma.