We then tested the expression of IgD, IgM and IgA in the main types of Breg populations in mice with MASLD (Fig. S3A–H) and another HCC mouse model, HCC/NRASG12V/p19Arf-/-, using multicolor FACS (Fig. S3I–P). This evidence concerns the gene CD40LG and metabolic dysfunction-associated steatotic liver disease.