Statistical differences were observed between the EMBE and EMBE+ENBD groups in nine factors: type of cancer (p<0.001), tumor location (p=0.026), preoperative AFP (p<0.001), gamma-glutamyl transpeptidase (p<0.001), aspartate amino transferase (p<0.001), TB (p=0.002), EPBD (p<0.001), RFA (p<0.001), and operative duration (p<0.001). The gene discussed is GGTLC1; the disease is cancer.