However, subsequent research has shown that normalizing 1,25(OH)2D3 levels could alleviate hearing loss in Klotho-deficient mice, appearing that hearing impairment might be indirectly mediated by high systemic 1,25(OH)2D3 rather than by a direct lack of Klotho expression (Carpinelli et al., 2011). This evidence concerns the gene KL and Hearing impairment.