Interestingly, while scRNA-seq of Ascl1-OE tumor cells demonstrates that some of the top most upregulated DEGs are associated with cancer metastases and invasion (Crip1, Gfap, Sparcl1, Tmsb4x, Apoe, Ier2, Gadd45g, Sparc, Cpe, Fabp5, Cdk2ap1, Jpt1, Vim, Ckb), and thus may contribute to the highly aggressive and diffused phenotype of these tumors, there was no significant change in Rnd3 expression, a direct target of ASCL1 (Supplementary Data 2) that has been shown to be important for neuronal migration36. This evidence concerns the gene APOE and neoplasm.