ASCL1 and neoplasm: To avoid possible confounding transcriptomes from non-tumor cells (i.e., reactive astrocytes, microglia/macrophages, neurons), scRNA-seq was performed only on FAC-sorted tdTOM+ cells of control tumors (N = 3, 18,163 cells) or GFP+ cells of Ascl1-OE tumors (N = 3, 28,109 cells) (Fig. 6b; Supplementary Fig. 6).