Gene expression analyses across these cell clusters confirmed that Ascl1/GFP, along with ASCL1 canonical NOTCH signaling target genes (Dll3, Notch1, Hes5) and the bHLH E-protein co-binding partners (Tcf4, Tcf12) were significantly elevated in Ascl1-OE tumor cells compared to control (Fig. 6g–l). This evidence concerns the gene TCF12 and neoplasm.