ASCL1 and neoplasm: Moreover, despite the shared binding of ASCL1 and OLIG2 to signature genes of both NSC/astroglial and OPC/oligodendroglial lineages, scRNA-seq of Ascl1-OE tumor cells revealed an upregulation of NSC/astrocyte signature genes but a downregulation of OPC/oligodendrocyte lineage genes compared to control tumors (Figs. 7, 8).