It has been found that in kidney cancer cells 786-O and SN12C, PBRM1 deficiency not only increases the levels of Akt and mTOR in the cells and their phosphorylated forms, activates AKT-mTOR signaling, but also increases the expression of mRNA and protein, which are key enzymes in glycolysis, such as phosphofructokinase (PFKP), enolase (ENO1), pyruvate kinase (PKM), and LDHA. The gene discussed is PBRM1; the disease is kidney cancer.