Addressing HSPB6's scarce presence in BLCA, we employed an overexpression lentivirus from Shanghai Jikai Gene Chemical Technology Co., Ltd to achieve stable HSPB6 upregulation in BLCA cells, verified in Fig. S1, B and C. Subsequent CCK-8 assays demonstrated that HSPB6 elevation significantly curtailed T24 and RT-112 cell proliferation (Fig. 2, A and B). The gene discussed is HSPB6; the disease is bladder transitional cell carcinoma.