The importance of VPS37A UEVL in autophagy was further verified using VPS37A KO U-2 OS human osteosarcoma cells (Figure S1F) in which the level of HT-GFP processing during autophagy was also found to be comparable to that in LC3 conjugation-defective ATG5 KO cells (Figure S1G). This evidence concerns the gene MAP1LC3A and osteosarcoma.