To enable the comparative study of DNA methylation maintenance dynamics supported by UHRF1 and DNMT1, we engineered HCT116 human colorectal carcinoma cells to express dox-inducible shRNAs targeting the UHRF1 or DNMT1 3′UTRs (Figure 1A and Supplementary Figure S1A). This evidence concerns the gene DNMT1 and colorectal carcinoma.