EGR1 and myopia: Up-regulated egr1 expression in 2-day normal-light-reared (∼4 wpf) efemp12C-Cas9 fish showed evidence myopia suppression, whereas down-regulation of egr1 in 4-week normal-light-reared (equivalent to 8 wpf) efemp12C-Cas9 fish showed a myopic shift (Fig. 6C).