We further utilize gene expression datasets of the human hippocampus (GSE28146, GSE29378, GSE36980, GSE48350, and GSE5281) to establish ROC curves to validate six core targets of MAPK8, CTNNB1, NFKB1, EGFR, BCL2, and NFE2L2 related to AD in diagnosis, as shown in Figure 8. This evidence concerns the gene MAPK8 and Alzheimer disease.