CFTR and cystic fibrosis: Among them, given that the most prevalent cystic fibrosis-causing mutant F508del destabilizes the CFTR channel by affecting the least-stable noncovalent interaction, which was closely related to the C-terminal region near the Mg/ATP site at the interface of NBD1 and NBD2, enhancing the interdomain interface is necessary for rescuing the thermal defect in F508del-hCFTR.