In addition, these data sets show a strong positive correlation between AIRE expression and markers of TCR signaling such as Zap70 and IFN-γ (Supplemental Fig. 1B) — again consistent with our studies showing that increased AIRE expression in melanomas induces strong indicators of T cell activation and breaking of tolerance (Figs. 2E&F, 3D and 6E). This evidence concerns the gene ZAP70 and melanoma.