Although similar numbers of anti-B16-F10 CD8+ T cells were generated by AAV-8-AIRE and AAV-8-AIRE + anti-PD-1 ICB (by ELISPOT), the activity of those CD8+ T cells (amount of IFN-γ/CD8+ T cell) was significantly greater with addition of anti-PD-1 ICB (Fig. 6E) — showing the importance of de-repression of exhausted anti-tumour T cells by the anti-PD-1 treatment22, 23, 24, 31. The gene discussed is IFNG; the disease is neoplasm.