Changes in the total numbers of epitopes from the well characterized melanoma TAA TYRP1 between B16-F10 cell lines expressing different levels of AIRE (Fig. 7C) reflected the overall data set (Fig. 7B) in that over-expression of AIRE in B16-F10 cells increased the total number of TYRP1 epitopes, whilst knock down of AIRE dramatically decreased the number of TYRP1 epitopes (Fig. 7C). The gene discussed is TYRP1; the disease is melanoma.