Considering the 25 % VAF for IDH1, IDH2, pTERT C228 and pTERT C250, significant (t-test; p ≤ 0.05) differences between Wt samples and tumor marker samples were identified with fewer than 80 sequences per marker (Sequencing performed on a Flongle R9.4.1 flow cell, IDH1 – 45 Seq, 00:45 ± 00:03 min; IDH2 – 14 Seq, 00:18 ± 00:12 min; pTERT C228 – 73 Seq, 38:30 ± 17:41 min; pTERT C250 – 77 Seq, 37:34 ± 18:58 min; Fig. 2). This evidence concerns the gene IDH1 and neoplasm.