Tumor pieces at approximately 2 × 2 × 2 mm3 from an HNSCC patient containing amplified or diploid MAP3K13 were implanted subcutaneously with Matrigel (Corning) in the mice according to the SOP50101 Implantation and Cryopreservation of Tissue for PDX Generation protocol from the NIH Patient-Derived Models Repository (PDMR). The gene discussed is MAP3K13; the disease is head and neck squamous cell carcinoma.