Colony formation assays validated tumor suppressor function of SEMA6B, MT1E, S100A, and ASAH2 from the Perturb-DBiT top ten enriched hits (Figures 4A, S4C).This was exemplified by the inclusion of four previously reported tumor suppressors (ARHGAP6, CREBZF, DHRS7C, and NR4A3)54–57, whose corresponding sgRNAs were abundantly detected by Perturb-DBiT. Here, ARHGAP6 is linked to neoplasm.