MT1E and neoplasm: Intriguingly, the distribution patterns of the sgRNAs for SEMA6B, MT1E, and S100A4 - three of the four validated tumor suppressors-align well with the tumor pseudotime trajectory, suggesting the formation of diverse tumor genotypes in a single pooled screen that can recapitulate the whole epectrum of tumor evolution, from regions with fewer sgRNAs to colonies with substantial sgRNA enrichment (Figures 4E, 4F).