P‐selectin consists of 789 amino acid residues and is structured into three parts: N‐terminal extracellular domain (730 amino acid residues) contains a lectin‐like domain, an epidermal growth factor like domain and nine complement regulatory protein consensus repeat units; Transmembrane region is composed of 24 amino acid residues; C‐terminal intracellular domain contains 35 amino acid residues.[21] Silencing P‐selectin significantly decreased the growth of colony spheroids, migration as well invasion in ICC‐TRCs (Figure S16 (Supporting Information); and Figure4A,B). This evidence concerns the gene EGF and intrahepatic cholangiocarcinoma.