PTEN and neoplasm: We established NTT tumours and RTT tumours of the YUMM1.7 mouse melanoma model (BrafV600E, Pten–/–Cdkn2a–/–) that express the model antigen ovalbumin (OVA) in Rag2–/– mice and performed ACT by intravenously injecting activated tumour antigen-specific CD8+ T cells (OT-1Luc) (Fig. 1a).