Initially, lentiviral CRISPR-Cas9 technology was employed to knockout YY1 in multiple EGFR-mutant, KRAS-altered, or ALK-rearranged lung cancer cell lines using two independent sgRNAs (Fig. 4A), and cell viability was significantly inhibited without exception (Fig. 4B). The gene discussed is KRAS; the disease is lung carcinoma.