To test the role of Tmem163 in aging-related Zn2+ accumulation in the spinal dorsal horn and aging-related itch, we generated Tmem163 conditional knockout (cKO) mice in which Tmem163 was selectively deleted in VGluT1+ DRG neurons (Fig 3G and 3H). This evidence concerns the gene SLC17A7 and Pruritus.