Considering evolutionary relationship among Srp1/Tip1-family members, we constructed the tir2-5 quadruple mutant strain, awp6-7 double mutant strain, and the sextuple mutant (tir2-5 awp6-7) in which all Srp1/Tip1-family genes that are upregulated during infection (TIR2-5 and AWP6-7) were deleted. This evidence concerns the gene TAX1BP3 and infection.