To clarify the regulatory mechanisms by which TCF7L2 and NOTCH2 control downstream genes in the classical Wnt pathway, we initially knocked down TCF7L2 and NOTCH2 in CRC organoids (Figure S5A,B, Supporting Information), which decreased the expression levels of MYC, JUN, FOSL1, CCND1, CCND2, and CCND3 (Figure 5C). This evidence concerns the gene NOTCH2 and colorectal carcinoma.