RNA‐seq and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the P2 and LM8 organoids showed enhanced transcriptional regulation of the Wnt and other tumor‐associated pathways, especially the Notch and Jak/Stat pathways, in the medium without RSPO1 (Figure 1K). The gene discussed is SOAT1; the disease is neoplasm.