Furthermore, when cultured in medium without RSPO1, organoids with high transcriptional levels of the Wnt pathway not only showed higher transcriptional activity of the Wnt pathway, but also activation of some pathways involved in tumor development, such as the Notch, Jak/Stat, and Hedgehog pathways.[34, 35, 36] Maia Chanrion et al. The gene discussed is RSPO1; the disease is neoplasm.