Figure 5B shows that residues Val201, Thr355 and Tyr359 of HTR1A formed three conventional hydrogen bonds with TVGF. Simultaneously, there existed alkyl interactions between TVGF and HTR1A (Val200 and Met337). Insomnia is closely related to depression, and MAOA is a key enzyme involved in the degradation of monoamine neurotransmitters and plays an important role in depression (Wang et al., 2017). As shown in Figure 5C, residues Thr205 and Asp132 of MAOA formed conventional hydrogen bonds with TVGF, while Gly110 formed carbon hydrogen bonds. This evidence concerns the gene HTR1A and major depressive disorder.