Simultaneous observation of Annexin V-FITC/PI staining results revealed that the low, medium, and high doses of BBR administration groups significantly induced apoptosis in RA-FLS cells (green fluorescence for early apoptotic cells and red + green for late apoptotic cells) compared with the corresponding control cells and showed a dose-dependent trend, which was in line with the trend of the results of flow cytometry experiments (Figure 2C). This evidence concerns the gene ANXA5 and rheumatoid arthritis.