Overexpression of SIRT1 in BMECs rescued claudin-5, occludin, ZO-1, and VE-cadherin expression, while stabilizing claudin-5/ZO-1 interactions to protect against senescence-induced brain endothelial barrier hyperpermeability, cerebral ischemia, and OGD/R [59,63,69]. The gene discussed is CDH5; the disease is brain ischemia.