However, phytochemical characterization is an ongoing study of V. ponticum to detect and isolate bioactive substances alongside further molecular-based analysis involving annexin V-FITC staining; caspase activity and employing mitochondrial pharmacology tests will be incorporated in subsequent studies with the isolated bioactive compound, to further validate the effectiveness of the V. ponticum cytotoxicity on NSCLC cells. The gene discussed is ANXA5; the disease is non-small cell lung carcinoma.