In this study, the authors found that all these substances and their respective re-complexes, tested in vitro by performing the real-time quaking-induced conversion assay with recombinant PrP seeded with cerebrospinal fluid from CJD patients, blocked de novo abnormal formation and aggregation of the amyloid-like abnormal prion protein PrPSc [25] (Figure 2d). The gene discussed is PRNP; the disease is Creutzfeldt Jacob disease.