This work aimed to study and compare the gene expression associated with cell motility from an experimental breast cancer model induced by radiation and estrogen, including a disintegrin and metallopeptidase 12 gene (ADAM12), the cysteine-rich angiogenic inducer 61 gene (CYR61), the fibronectin leucine rich transmembrane protein 2 gene (FLRT2), the slit guidance ligand 2 gene (SLIT2), the vanin-1 gene (VNN1), the myosin light chain kinase gene (MYLK), the microtubule-associated protein 1B gene (MAP1B), and the tubulin alpha 1a gene (TUBA1A). This evidence concerns the gene FLRT2 and breast cancer.