To assess the impact of brain ischemia on the expression of AQP4 and GDNF in EV cargo, we analyzed these proteins’ band intensities in the TEVs and ADEVs of AIS patients (D1, D7: n = 18, M1: n = 12) and compared them to those of the control subjects (n = 9). Here, GDNF is linked to androgen insensitivity syndrome.