GDNF and squamous cell carcinoma: Similarly, previous studies reported multiple bands on the GDNF blots: three major bands in head and neck squamous cell carcinoma cell lines (24 kDa as the predicted MW, 35 kDa as a probable glycosylated form, and a band right above 10 kDa as a signal peptide of GDNF) [55], two bands in human limbal epithelia (21 kDa as a mature form and 35 kDa as a glycosylated homodimer of GDNF) [54], as well as two bands in lysates from adult and neonatal mouse brains (≈36 kDa as fully glycosylated mature homodimers and ≈50 kDa as a proform homodimer of GDNF) [56].