Furthermore, double RAB27A and RAB27B KO in the A375 cell line did not lead to the inhibition of the number of sEVs released into the culture medium; thus, Rab27B did not compensate for the loss of Rab27A in these melanoma cells, even though Rab27B expression was enhanced significantly in RAB27A KO. Here, RAB27B is linked to melanoma.