In this study, 6M-4MC was shown to activate melanin production in B16F10 melanoma cells by upregulating tyrosinase, TRP-1, and TRP-2, indicating that 6M-4MC may stimulate intracellular tyrosinase activity by enhancing the expression of these melanogenesis-related enzymes (Figure 5). The gene discussed is TYR; the disease is melanoma.